Mathematical model for the reconstituted system with PGK, GAPDH, TPI and FBPAase.
SEEK ID: http://localhost:3000/assays/2
Modelling analysis
Projects: Default Project
Investigation: Central Carbon Metabolism of Sulfolobus solfataricus
Study: Carbon loss at high T
Assay position:
Biological problem addressed: Model Analysis Type
Organisms: No organisms
Data:
- Metabolite concentrations during reconstituted enzyme incubation (Validation data)
- Model simulation and Exp data for reconstituted system (Validation data)
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Creators and SubmitterViews: 10
Created: 8th May 2026 at 15:58
Last updated: 8th May 2026 at 15:58
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Projects: Default Project
Institutions: Default Institution
This is a test programme for the SEEK sandbox.
Projects: Default Project, openEO - a common, open source interface between Earth Observation data infrastructures and front-end applications, JAEGER, FDIUSER1, FDIUSER2
Web page: http://www.seek4science.org
A description for the default project
Programme: Default Programme
Public web page: https://www.seek4science.org
Organisms: Sulfolobus solfataricus
An investigation in the CCM of S. solfataricus with a focus on the unique temperature adaptations and regulation; using a combined modelling and experimental approach.
Submitter: Guest User
Studies: Carbon loss at high T
Assays: Assay stream, Model reconstituted system, Reconstituted system reference state
Snapshots: No snapshots
The carbon loss at high T description will be here but I am currently not imaginative enough.
Submitter: Guest User
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Assays: Assay stream, Model reconstituted system, Reconstituted system reference state
Snapshots: No snapshots
Experimental data for the reconstituted system are plotted together with the model prediction.
Investigations: Central Carbon Metabolism of Sulfolobus solfata...
Studies: Carbon loss at high T
Assays: Model reconstituted system, Reconstituted system reference state
The purified enzymes, PGK, GAPDH, TPI and FBPAase were incubated at 70 C en conversion of 3PG to F6P was followed.
Investigations: Central Carbon Metabolism of Sulfolobus solfata...
Studies: Carbon loss at high T
Assays: Model reconstituted system, Reconstituted system reference state
The PGK, GAPDH, TPI and FBPAase were modelled together using the individual rate equations. Closed system.
Creator: Guest User
Submitter: Guest User
Model type: Ordinary differential equations (ODE)
Model format: SBML
Environment: JWS Online
Organism: Sulfolobus solfataricus
Investigations: Central Carbon Metabolism of Sulfolobus solfata...
Studies: Carbon loss at high T
Assays: Model reconstituted system
Standard operating procedure for reconstituting the gluconeogenic enzyme system from Sulfolobus solfataricus to study metabolic pathway efficiency at high temperatures.
Creator: Guest User
Submitter: Guest User
Investigations: Central Carbon Metabolism of Sulfolobus solfata...
Studies: Carbon loss at high T
Assays: Model reconstituted system, Reconstituted system reference state
Abstract (Expand)
Authors: T. Kouril, D. Esser, J. Kort, H. V. Westerhoff, B. Siebers, J. Snoep
Date Published: 8th May 2026
Publication Type: Journal Article
PubMed ID: 23865479
Citation: Kouril, T. et al. Intermediate instability at high temperature leads to low pathway efficiency for an in vitro reconstituted system of gluconeogenesis in Sulfolobus solfataricus. FEBS J. 2015;687:100-108.
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