Reconstituted Enzyme System Protocol Version 1
Standard operating procedure for reconstituting the gluconeogenic enzyme system from Sulfolobus solfataricus to study metabolic pathway efficiency at high temperatures.
SEEK ID: http://localhost:3000/sops/1?version=1
Filename: test_sop.txt
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Created: 8th May 2026 at 15:58
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Gluconeogenesis in ...
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Version 1 (earliest) Created 8th May 2026 at 15:58 by Guest User
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Related items
Projects: Default Project
Institutions: Default Institution
This is a test programme for the SEEK sandbox.
Projects: Default Project, openEO - a common, open source interface between Earth Observation data infrastructures and front-end applications, JAEGER, FDIUSER1, FDIUSER2
Web page: http://www.seek4science.org
A description for the default project
Programme: Default Programme
Public web page: https://www.seek4science.org
An investigation in the CCM of S. solfataricus with a focus on the unique temperature adaptations and regulation; using a combined modelling and experimental approach.
Submitter: Guest User
Studies: Carbon loss at high T
Assays: Assay stream, Model reconstituted system, Reconstituted system reference state
The carbon loss at high T description will be here but I am currently not imaginative enough.
Submitter: Guest User
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Assays: Assay stream, Model reconstituted system, Reconstituted system reference state
The four purified enzymes were incubated in assay buffer and consumption of 3PG and production of F6P were measured in time, together with GAP and DHAP concentrations.
Submitter: Guest User
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Study: Carbon loss at high T
Mathematical model for the reconstituted system with PGK, GAPDH, TPI and FBPAase.
Submitter: Guest User
Biological problem addressed: Model Analysis Type
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Study: Carbon loss at high T
A collection of data files, models, SOPs and publications related to the reconstituted gluconeogenic enzyme system from Sulfolobus solfataricus.
