The carbon loss at high T description will be here but I am currently not imaginative enough.
SEEK ID: http://localhost:3000/studies/1
Central Carbon Metabolism of Sulfolobus solfataricus
Projects: Default Project
Study position:
Export PNG
Creators and SubmitterViews: 9
Created: 8th May 2026 at 15:58
Related items
- People (1)
- Programmes (1)
- Projects (1)
- Investigations (1)
- Observation Units (1)
- Assays (3)
- Data files (2)
- Models (1)
- SOPs (1)
- Publications (1)
- Sample types (2)
- Samples (6)
Projects: Default Project
Institutions: Default Institution
This is a test programme for the SEEK sandbox.
Projects: Default Project, openEO - a common, open source interface between Earth Observation data infrastructures and front-end applications, JAEGER, FDIUSER1, FDIUSER2
Web page: http://www.seek4science.org
A description for the default project
Programme: Default Programme
Public web page: https://www.seek4science.org
Organisms: Sulfolobus solfataricus
An investigation in the CCM of S. solfataricus with a focus on the unique temperature adaptations and regulation; using a combined modelling and experimental approach.
Submitter: Guest User
Studies: Carbon loss at high T
Assays: Assay stream, Model reconstituted system, Reconstituted system reference state
Snapshots: No snapshots
The four purified enzymes were incubated in assay buffer and consumption of 3PG and production of F6P were measured in time, together with GAP and DHAP concentrations.
Submitter: Guest User
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Study: Carbon loss at high T
Organisms: Sulfolobus solfataricus
SOPs: Reconstituted Enzyme System Protocol
Data files: Metabolite concentrations during reconstituted ..., Model simulation and Exp data for reconstituted...
Snapshots: No snapshots
Mathematical model for the reconstituted system with PGK, GAPDH, TPI and FBPAase.
Submitter: Guest User
Biological problem addressed: Model Analysis Type
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Study: Carbon loss at high T
Organisms: No organisms
Models: Mathematical model for the combined four enzyme...
SOPs: Reconstituted Enzyme System Protocol
Data files: Metabolite concentrations during reconstituted ..., Model simulation and Exp data for reconstituted...
Snapshots: No snapshots
A stream of assays? This is a test assay stream for the example data.
Submitter: Guest User
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Study: Carbon loss at high T
Organisms: No organisms
SOPs: No SOPs
Data files: No Data files
Snapshots: No snapshots
Experimental data for the reconstituted system are plotted together with the model prediction.
Creators: Admin User, Person A, Person B
Submitter: Guest User
Investigations: Central Carbon Metabolism of Sulfolobus solfata...
Studies: Carbon loss at high T
Assays: Model reconstituted system, Reconstituted system reference state
The purified enzymes, PGK, GAPDH, TPI and FBPAase were incubated at 70 C en conversion of 3PG to F6P was followed.
Creator: Guest User
Submitter: Guest User
Investigations: Central Carbon Metabolism of Sulfolobus solfata...
Studies: Carbon loss at high T
Assays: Model reconstituted system, Reconstituted system reference state
The PGK, GAPDH, TPI and FBPAase were modelled together using the individual rate equations. Closed system.
Creator: Guest User
Submitter: Guest User
Model type: Ordinary differential equations (ODE)
Model format: SBML
Environment: JWS Online
Organism: Sulfolobus solfataricus
Investigations: Central Carbon Metabolism of Sulfolobus solfata...
Studies: Carbon loss at high T
Assays: Model reconstituted system
Standard operating procedure for reconstituting the gluconeogenic enzyme system from Sulfolobus solfataricus to study metabolic pathway efficiency at high temperatures.
Creator: Guest User
Submitter: Guest User
Investigations: Central Carbon Metabolism of Sulfolobus solfata...
Studies: Carbon loss at high T
Assays: Model reconstituted system, Reconstituted system reference state
Abstract (Expand)
Authors: T. Kouril, D. Esser, J. Kort, H. V. Westerhoff, B. Siebers, J. Snoep
Date Published: 8th May 2026
Publication Type: Journal Article
PubMed ID: 23865479
Citation: Kouril, T. et al. Intermediate instability at high temperature leads to low pathway efficiency for an in vitro reconstituted system of gluconeogenesis in Sulfolobus solfataricus. FEBS J. 2015;687:100-108.
Sample type for purified enzyme preparations used in reconstituted systems
Enzyme Name (String) *, EC Number (String) , Concentration (Real number) ( mg/ml ) *, Specific Activity (Real number) , Storage Temperature (Integer) ( °C ) , Purification Steps (Integer)
Sample type for bacterial culture experiments related to thermophile studies
Culture Name (String) *, Strain Used (String) *, Growth Temperature (Real number) ( °C ) *, Culture Volume (Real number) ( mL ) , pH (Real number) , Growth Phase Complete (Boolean)
Actions
Order Assays
Download