Central Carbon Metabolism of Sulfolobus solfataricus

An investigation in the CCM of S. solfataricus with a focus on the unique temperature adaptations and regulation; using a combined modelling and experimental approach.

Submitter: Guest User

Studies: Carbon loss at high T

Assays: Assay stream, Model reconstituted system, Reconstituted system reference state

Carbon loss at high T

The carbon loss at high T description will be here but I am currently not imaginative enough.

Submitter: Guest User

Investigation: Central Carbon Metabolism of Sulfolobus solfata...

Assays: Assay stream, Model reconstituted system, Reconstituted system reference state

Reconstituted Enzyme System Protocol

Standard operating procedure for reconstituting the gluconeogenic enzyme system from Sulfolobus solfataricus to study metabolic pathway efficiency at high temperatures.

Creator: Guest User

Submitter: Guest User

Intermediate instability at high temperature leads to low pathway efficiency for an in vitro reconstituted system of gluconeogenesis in Sulfolobus solfataricus

Abstract (Expand)

Four enzymes of the gluconeogenic pathway in Sulfolobus solfataricus were purified and kinetically characterized. The enzymes were reconstituted in vitro to quantify the contribution of temperature … instability of the pathway intermediates to carbon loss from the system. The reconstituted system, consisting of phosphoglycerate kinase, glyceraldehyde 3-phosphate dehydrogenase, triose phosphate isomerase and the fructose 1,6-bisphosphate aldolase/phosphatase, maintained a constant consumption rate of 3-phosphoglycerate and production of fructose 6-phosphate over a 1-h period. Cofactors ATP and NADPH were regenerated via pyruvate kinase and glucose dehydrogenase. A mathematical model was constructed on the basis of the kinetics of the purified enzymes and the measured half-life times of the pathway intermediates. The model quantitatively predicted the system fluxes and metabolite concentrations. Relative enzyme concentrations were chosen such that half the carbon in the system was lost due to degradation of the thermolabile intermediates dihydroxyacetone phosphate, glyceraldehyde 3-phosphate and 1,3-bisphosphoglycerate, indicating that intermediate instability at high temperature can significantly affect pathway efficiency.

Authors: T. Kouril, D. Esser, J. Kort, H. V. Westerhoff, B. Siebers, J. Snoep

Date Published: 8th May 2026

Publication Type: Journal Article

Gluconeogenesis in Sulfolobus solfataricus

A collection of data files, models, SOPs and publications related to the reconstituted gluconeogenic enzyme system from Sulfolobus solfataricus.

Maintainers: Guest User

Number of items: 7

Tags: gluconeogenesis, thermophile, metabolism

Experimental setup for the reconstituted gluconeogenic enzyme system

This document describes the experimental setup and procedures used for reconstituting the gluconeogenic enzyme system from Sulfolobus solfataricus.

Creator: Admin User

Submitter: Guest User

Bacterial Culture

Sample type for bacterial culture experiments related to thermophile studies

Culture Name (String) *, Strain Used (String) *, Growth Temperature (Real number) ( °C ) *, Culture Volume (Real number) ( mL ) , pH (Real number) , Growth Phase Complete (Boolean)

bacterial culture, thermophile, Microbiology

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