A description for the default project
Programme: Default Programme
SEEK ID: http://localhost:3000/projects/1
Public web page: https://www.seek4science.org
Organisms: Sulfolobus solfataricus
FAIRDOM PALs: Guest User
Project created: 8th May 2026
Related items
- People (2)
- Programmes (1)
- Institutions (1)
- Investigations (2+2)
- Studies (2)
- Observation Units (1)
- Assays (4)
- Strains (1)
- Data files (3)
- Models (1)
- SOPs (1)
- Publications (1)
- Presentations (1)
- Events (1)
- Documents (1)
- Sample types (2)
- Samples (6)
- Collections (1)
Projects: Default Project, BEANFORMER, openEO - a common, open source interface between Earth Observation data infrastructures and front-end applications, BERGMANN, BLENDER, COLDEWEY, EFFENBERGER, HAUFF, JAEGER, CJAEGER, KEBEL, KÜNNE, KUTZERA, NAGEL, SELKRIG, NUXOLL, PETZOLD, PLATTE, SCHOEPS, SCHULZE, FDIUSER1, FDIUSER2
Institutions: Default Institution, Leipzig University, ttt, DKMS Life Science Lab, in.vent Diagnostica, RWTH Aachen, medizinische Fakultät, IDG Institut für digitale Gesundheitsdaten RLP gGmbH, Universitätsklinikum Schleswig-Holstein, Bernhard Nocht Institute for Tropical Medicine, Martin-LutherUniversität HalleWittenberg, Otto-von-Guericke, Universitätsmedizin Halle, Bundesanstalt für, Universitätsklinikum Aachen, University of Lübeck, DKMS Group gGmbH CTU, Hochschule Mittweida, IDG RLP, Medizinische, IMISE
https://orcid.org/0000-0002-1825-0097
Expertise: administration, Data Management
Tools: SEEK, Ruby on Rails
Projects: Default Project
Institutions: Default Institution
This is a test programme for the SEEK sandbox.
Projects: Default Project, openEO - a common, open source interface between Earth Observation data infrastructures and front-end applications, JAEGER, FDIUSER1, FDIUSER2
Web page: http://www.seek4science.org
ROR ID: Not specified
Department: Not specified
Country:
United Kingdom
City: Manchester
Web page: Not specified
Investigation into COVID-19 patient outcomes in hospitalised adults
Submitter: Admin User
Studies: Hospitalised COVID-19 Patients Cohort
Assays: Patient Registry Data Collection
Snapshots: No snapshots
An investigation in the CCM of S. solfataricus with a focus on the unique temperature adaptations and regulation; using a combined modelling and experimental approach.
Submitter: Guest User
Studies: Carbon loss at high T
Assays: Assay stream, Model reconstituted system, Reconstituted system reference state
Snapshots: No snapshots
Observational cohort study of adults hospitalised with confirmed COVID-19
Submitter: Admin User
Investigation: COVID-19 Clinical Research
Assays: Patient Registry Data Collection
Snapshots: No snapshots
The carbon loss at high T description will be here but I am currently not imaginative enough.
Submitter: Guest User
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Assays: Assay stream, Model reconstituted system, Reconstituted system reference state
Snapshots: No snapshots
The four purified enzymes were incubated in assay buffer and consumption of 3PG and production of F6P were measured in time, together with GAP and DHAP concentrations.
Submitter: Guest User
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Study: Carbon loss at high T
Organisms: Sulfolobus solfataricus
SOPs: Reconstituted Enzyme System Protocol
Data files: Metabolite concentrations during reconstituted ..., Model simulation and Exp data for reconstituted...
Snapshots: No snapshots
Mathematical model for the reconstituted system with PGK, GAPDH, TPI and FBPAase.
Submitter: Guest User
Biological problem addressed: Model Analysis Type
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Study: Carbon loss at high T
Organisms: No organisms
Models: Mathematical model for the combined four enzyme...
SOPs: Reconstituted Enzyme System Protocol
Data files: Metabolite concentrations during reconstituted ..., Model simulation and Exp data for reconstituted...
Snapshots: No snapshots
A stream of assays? This is a test assay stream for the example data.
Submitter: Guest User
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Study: Carbon loss at high T
Organisms: No organisms
SOPs: No SOPs
Data files: No Data files
Snapshots: No snapshots
Data collection assay for the COVID-19 patient registry
Submitter: Admin User
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: COVID-19 Clinical Research
Submitter: Not available
Provider Name: BacDive
Provider's strain ID: 123456789
Organism: Sulfolobus solfataricus
Genotypes: wild-type
Phenotypes: wild-type
Comment: This is a test strain.
Clinical data of hospitalised COVID-19 patients across European hospitals.
Creators: None
Submitter: Admin User
Investigations: COVID-19 Clinical Research
Studies: Hospitalised COVID-19 Patients Cohort
Assays: Patient Registry Data Collection
Experimental data for the reconstituted system are plotted together with the model prediction.
Creators: Admin User, Person A, Person B
Submitter: Guest User
Investigations: Central Carbon Metabolism of Sulfolobus solfata...
Studies: Carbon loss at high T
Assays: Model reconstituted system, Reconstituted system reference state
The purified enzymes, PGK, GAPDH, TPI and FBPAase were incubated at 70 C en conversion of 3PG to F6P was followed.
Creator: Guest User
Submitter: Guest User
Investigations: Central Carbon Metabolism of Sulfolobus solfata...
Studies: Carbon loss at high T
Assays: Model reconstituted system, Reconstituted system reference state
The PGK, GAPDH, TPI and FBPAase were modelled together using the individual rate equations. Closed system.
Creator: Guest User
Submitter: Guest User
Model type: Ordinary differential equations (ODE)
Model format: SBML
Environment: JWS Online
Organism: Sulfolobus solfataricus
Investigations: Central Carbon Metabolism of Sulfolobus solfata...
Studies: Carbon loss at high T
Assays: Model reconstituted system
Standard operating procedure for reconstituting the gluconeogenic enzyme system from Sulfolobus solfataricus to study metabolic pathway efficiency at high temperatures.
Creator: Guest User
Submitter: Guest User
Investigations: Central Carbon Metabolism of Sulfolobus solfata...
Studies: Carbon loss at high T
Assays: Model reconstituted system, Reconstituted system reference state
Abstract (Expand)
Authors: T. Kouril, D. Esser, J. Kort, H. V. Westerhoff, B. Siebers, J. Snoep
Date Published: 8th May 2026
Publication Type: Journal Article
PubMed ID: 23865479
Citation: Kouril, T. et al. Intermediate instability at high temperature leads to low pathway efficiency for an in vitro reconstituted system of gluconeogenesis in Sulfolobus solfataricus. FEBS J. 2015;687:100-108.
Four enzymes of the gluconeogenic pathway in Sulfolobus solfataricus were purified and kinetically characterized. The enzymes were reconstituted in vitro to quantify the contribution of temperature instability of the pathway intermediates to carbon loss from the system. The reconstituted system, consisting of phosphoglycerate kinase, glyceraldehyde 3-phosphate dehydrogenase, triose phosphate isomerase and the fructose 1,6-bisphosphate aldolase/phosphatase, maintained a constant consumption rate ...
Creator: Guest User
Submitter: Guest User
Event for publication
Start Date: 8th May 2026
End Date: 9th May 2026
Event type: Not specified
This document describes the experimental setup and procedures used for reconstituting the gluconeogenic enzyme system from Sulfolobus solfataricus.
Creator: Admin User
Submitter: Guest User
Investigations: No Investigations
Studies: No Studies
Assays: No Assays
Sample type for purified enzyme preparations used in reconstituted systems
Enzyme Name (String) *, EC Number (String) , Concentration (Real number) ( mg/ml ) *, Specific Activity (Real number) , Storage Temperature (Integer) ( °C ) , Purification Steps (Integer)
Sample type for bacterial culture experiments related to thermophile studies
Culture Name (String) *, Strain Used (String) *, Growth Temperature (Real number) ( °C ) *, Culture Volume (Real number) ( mL ) , pH (Real number) , Growth Phase Complete (Boolean)
A collection of data files, models, SOPs and publications related to the reconstituted gluconeogenic enzyme system from Sulfolobus solfataricus.
Overview
Tags